Cours de biochimie structurale: Lipides et dérivés isopréniques, Volume 4. Front Cover. Pierre Louisot. Simep éditions, – 93 pages. Cours de biochimie. Biochimie Métabolique: Cours de Cycle de Krebs Cours de la physiologie cardiaque et lymphatique svi s5 Cours de Biochimie structurale svi s3. بسم الله . Spécialité: Biochimie structurale. Présenté par. Guillaume sur l’étude de la paraoxonase humaine hPON1 au cours du service militaire du. Pr. Eric Chabrière.

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Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells

Oxford University Press is a department of the University of Oxford. Remarkably, a shift between the theoretical and experimental molecular masses of Usp45, 45 vs.

Binding and cross-linking properties of galectins. Putative O -glycosylation sites and a membrane anchor are necessary for apical delivery of the coure neurotrophin receptor in Caco-2 cells. Close mobile search navigation Article navigation.

Polarized epithelial membrane traffic: To enter the program, students must have a solid background in biology, and a good command of spoken and written English for a direct application in the second year – M2 or English AND FRENCH for an application in the first year. Vectorial targeting of apical and basolateral plasma membrane proteins in a human adenocarcinoma epithelial cell line. This is the first direct demonstration for a role of a lectin in apical transport.

Serpin was degraded to different degrees by the four LAB strains, and might have interfered with the detection of bacterial proteins in this zone of the molecular mass. Galectin-4 was only present in P2, i. If possible, students should have also participated in an internship of at least two months in a research laboratory.


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O-linked glycans mediate apical sorting of human intestinal sucrase-isomaltase through association with lipid rafts. Immunostaining of unpermeabilized cells showed that galectin-4 was bound to the extracellular side of the coure membrane.

Proteomic analysis of cell surface-associated proteins from probiotic Lactobacillus plantarum. Results are expressed as molar ratio or percentage of molar ratio. For detergent extraction on living cells, the cells were incubated for 2 min in 10 mM Hepes, pH 7.

Inhibition of galectin-4 expression abrogates apical biochime To analyze whether galectin-4 had a role in the delivery of apical proteins, we depleted galectin-4 expression in HT 5M12 cells by RNA interference RNAi. The samples were processed as previously described Gouyer et al. Raft association plays a role in sorting to the apical surface Brown and Rose, ; Lipardi et al. Molecular and phenotypical characterization of Cronobacter species isolated with high occurrence from oats and linseeds.

Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate. Supernatants were collected and cells were then lysed in warm RIPA buffer 0.

Ayoub Alhyane shared a link. Functional rafts in cell membranes. Because secreted proteins were precipitated in the stationary phase of culture, cell lysis cannot be excluded, but at least it was minor.

Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells

S3, available at http: Online supplemental material Fig. Refined prognostic evaluation in colon carcinoma using immuno-histochemical structurals fingerprinting. Searches in databases were done by using the Profound software.

For example, a block at the level of TGN might still allow misloading of apical proteins for basolateral delivery but the fate of apical cargo might be different if the inhibition is exerted at a later step in the pathway. If the internship takes place in a laboratory belonging to another university, the student must either validate a “European” biochimke proposed by the ENS or validate a course proposed by the other university worth 6 ECTS credits, approved by the BioSciences Master pedagogical team.



VIP36, a lectin identified as a component of isolated carrier vesicles, has been implicated in sorting of proteins cojrs apical delivery Fiedler and Simons, There are many ways to apply, according to your curriculum and your nationality. Louis, MOand D.

Elective practical courses 3 ECTS. For this reason, post-translational modifications of such residues, like O -glycosylations, may be responsible for the mass shift, but this point needs further research.

Successful applicants must have a solid background in biology studies with an excellent understanding of written and spoken English AND French and will have preferably undertaken an internship of at least two months in a research laboratory.

Where externalization occurs is also not known.

These sorting motifs are recognized by adaptor complexes APs; Nakatsu and Ohno, Candidates whose applications have been selected will be contacted for an interview consisting of 15 minute discussion period. For unidentified bands, it seems that databases do not yet contain homologs of such proteins.